The aim of this paper is to determine the effects of particle equilibrium charge and face velocity on the particle filtration efficiency of non-woven filter and woven fabric. In this study, N95 respirator filter media and microfiber fabric were tested against sodium chloride (NaCl) and polystyrene Latex (PSL) particles in diameter of 0.3 µm. A neutralizer and an electrostatic size classifier were used to classify the size of aerosol particles and a particle counter was used for measuring the particle number concentration at the upstream and downstream of the sample materials. Experimental results showed that filtration efficiency of NaCl and PSL particles of woven fabric and non-woven filter decreased due to particle equilibrium charge state in the ranges from 0.57 to 7.84%. It can be seen that increasing the face velocity resulted in the increase of the particle filtration efficiency of woven fabric. For non-woven fabric, particle filtration efficiency decreased as face velocity rose.